Proteomic analysis of up-regulated proteins in human promonocyte cells expressing severe acute respiratory syndrome coronavirus 3C-like protease.
Identifieur interne : 003625 ( Main/Exploration ); précédent : 003624; suivant : 003626Proteomic analysis of up-regulated proteins in human promonocyte cells expressing severe acute respiratory syndrome coronavirus 3C-like protease.
Auteurs : Chien-Chen Lai [Taïwan] ; Ming-Jia Jou ; Shiuan-Yi Huang ; Shih-Wein Li ; Lei Wan ; Fuu-Jen Tsai ; Cheng-Wen LinSource :
- Proteomics [ 1615-9853 ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Cysteine endopeptidases, Facteur inducteur d'apoptose, Proteasome endopeptidase complex, Protéines virales.
- génétique : Virus du SRAS.
- métabolisme : Monocytes.
- Cellules cultivées, Humains, Protéome, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Apoptosis Inducing Factor, Cysteine Endopeptidases, Proteasome Endopeptidase Complex, Viral Proteins.
- chemical , chemistry : Proteome.
- genetics : SARS Virus.
- metabolism : Monocytes.
- Cells, Cultured, Electrophoresis, Gel, Two-Dimensional, Humans.
Abstract
The pathogenesis of severe acute respiratory syndrome coronavirus (SARS CoV) is an important issue for treatment and prevention of SARS. Previously, SARS CoV 3C-like protease (3CLpro) has been demonstrated to induce apoptosis via the activation of caspase-3 and caspase-9 (Lin, C. W., Lin, K. H., Hsieh, T. H., Shiu, S. Y. et al., FEMS Immunol. Med. Microbiol. 2006, 46, 375-380). In this study, proteome analysis of the human promonocyte HL-CZ cells expressing SARS CoV 3CLpro was performed using 2-DE and nanoscale capillary LC/ESI quadrupole-TOF MS. Functional classification of identified up-regulated proteins indicated that protein metabolism and modification, particularly in the ubiquitin proteasome pathway, was the main biological process occurring in SARS CoV 3CLpro-expressing cells. Thirty-six percent of identified up-regulated proteins were located in the mitochondria, including apoptosis-inducing factor, ATP synthase beta chain and cytochrome c oxidase. Interestingly, heat shock cognate 71-kDa protein (HSP70), which antagonizes apoptosis-inducing factor was shown to down-regulate and had a 5.29-fold decrease. In addition, confocal image analysis has shown release of mitochondrial apoptogenic apoptosis-inducing factor and cytochrome c into the cytosol. Our results revealed that SARS CoV 3CLpro could be considered to induce mitochondrial-mediated apoptosis. The study provides system-level insights into the interaction of SARS CoV 3CLpro with host cells, which will be helpful in elucidating the molecular basis of SARS CoV pathogenesis.
DOI: 10.1002/pmic.200600459
PubMed: 17407183
Affiliations:
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Le document en format XML
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<term>Cells, Cultured</term>
<term>Cysteine Endopeptidases (biosynthesis)</term>
<term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Humans</term>
<term>Monocytes (metabolism)</term>
<term>Proteasome Endopeptidase Complex (biosynthesis)</term>
<term>Proteome (chemistry)</term>
<term>SARS Virus (genetics)</term>
<term>Viral Proteins (biosynthesis)</term>
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<term>Cysteine endopeptidases (biosynthèse)</term>
<term>Facteur inducteur d'apoptose (biosynthèse)</term>
<term>Humains</term>
<term>Monocytes (métabolisme)</term>
<term>Proteasome endopeptidase complex (biosynthèse)</term>
<term>Protéines virales (biosynthèse)</term>
<term>Protéome ()</term>
<term>Virus du SRAS (génétique)</term>
<term>Électrophorèse bidimensionnelle sur gel</term>
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<term>Viral Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Proteome</term>
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<term>Facteur inducteur d'apoptose</term>
<term>Proteasome endopeptidase complex</term>
<term>Protéines virales</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
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<term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Humans</term>
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<term>Humains</term>
<term>Protéome</term>
<term>Électrophorèse bidimensionnelle sur gel</term>
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<front><div type="abstract" xml:lang="en">The pathogenesis of severe acute respiratory syndrome coronavirus (SARS CoV) is an important issue for treatment and prevention of SARS. Previously, SARS CoV 3C-like protease (3CLpro) has been demonstrated to induce apoptosis via the activation of caspase-3 and caspase-9 (Lin, C. W., Lin, K. H., Hsieh, T. H., Shiu, S. Y. et al., FEMS Immunol. Med. Microbiol. 2006, 46, 375-380). In this study, proteome analysis of the human promonocyte HL-CZ cells expressing SARS CoV 3CLpro was performed using 2-DE and nanoscale capillary LC/ESI quadrupole-TOF MS. Functional classification of identified up-regulated proteins indicated that protein metabolism and modification, particularly in the ubiquitin proteasome pathway, was the main biological process occurring in SARS CoV 3CLpro-expressing cells. Thirty-six percent of identified up-regulated proteins were located in the mitochondria, including apoptosis-inducing factor, ATP synthase beta chain and cytochrome c oxidase. Interestingly, heat shock cognate 71-kDa protein (HSP70), which antagonizes apoptosis-inducing factor was shown to down-regulate and had a 5.29-fold decrease. In addition, confocal image analysis has shown release of mitochondrial apoptogenic apoptosis-inducing factor and cytochrome c into the cytosol. Our results revealed that SARS CoV 3CLpro could be considered to induce mitochondrial-mediated apoptosis. The study provides system-level insights into the interaction of SARS CoV 3CLpro with host cells, which will be helpful in elucidating the molecular basis of SARS CoV pathogenesis.</div>
</front>
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<name sortKey="Jou, Ming Jia" sort="Jou, Ming Jia" uniqKey="Jou M" first="Ming-Jia" last="Jou">Ming-Jia Jou</name>
<name sortKey="Li, Shih Wein" sort="Li, Shih Wein" uniqKey="Li S" first="Shih-Wein" last="Li">Shih-Wein Li</name>
<name sortKey="Lin, Cheng Wen" sort="Lin, Cheng Wen" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<name sortKey="Tsai, Fuu Jen" sort="Tsai, Fuu Jen" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
<name sortKey="Wan, Lei" sort="Wan, Lei" uniqKey="Wan L" first="Lei" last="Wan">Lei Wan</name>
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<country name="Taïwan"><noRegion><name sortKey="Lai, Chien Chen" sort="Lai, Chien Chen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
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